Buchi Encapsulator B-390 Bedienungsanleitung

11593477 enEncapsulatorOperation ManualB-390

2 Safety10 B-390 Operation Manual, Version C NoticeRisk of instrument damage by wrong mains supply.• External mains supply must meet the voltage give

2 Safety11 B-390 Operation Manual, Version C2.6 General safety rulesResponsibility of the operatorThe head of laboratory is responsible for training

3 Technical data12 B-390 Operation Manual, Version C3 Technical dataThis chapter introduces the reader to the instrument and its specifications. It c

3 Technical data13 B-390 Operation Manual, Version C3.1.2 Standard accessoriesTable 3-2: Standard accessoriesSet of 8 single nozzles 11057918Set of

3 Technical data14 B-390 Operation Manual, Version C3.1.4 Recommended spare partsTable 3-4: Recommended spare partsProduct Order no.O-ring set for s

3 Technical data15 B-390 Operation Manual, Version C3.2 Technical dataTable 3-5: Technical data Encapsulator B-390Power consumption max. 150 WConnec

4 Description of function16 B-390 Operation Manual, Version C4 Description of functionThis chapter explains the basic working principle of the Encap

4 Description of function17 B-390 Operation Manual, Version CFigure 4-1: Schematic representation of the Encapsulator B-390a Pressure bottle Bead pr

4 Description of function18 B-390 Operation Manual, Version CThe main parts of the Encapsulator B-390 are the control unit, the bead producing unit,

5 Putting into operation19 B-390 Operation Manual, Version C5 Putting into operationThis chapter describes how the instrument has to be installed. I

5 Putting into operation20 B-390 Operation Manual, Version C5.2 Installing the Encapsulator B-390Place the instrument on the lab bench with convenie

5 Putting into operation21 B-390 Operation Manual, Version CInstallation of the air lineA 3 m air tube (2.6×4.0 mm) is included with each Encapsulato

5 Putting into operation22 B-390 Operation Manual, Version C5.4 Assembling of the bead producing unitThe bead producing unit is the central part of

5 Putting into operation23 B-390 Operation Manual, Version C5.6 ElectrodeThe electrode is part of the electrostatic dispersion unit. It is attached

5 Putting into operation24 B-390 Operation Manual, Version CFigure 5-7: Position of the electrode below the carrier plate Figure 5-8: Grounding the p

5 Putting into operation25 B-390 Operation Manual, Version C5.7 Pressure bottleThe pressure bottle is an autoclavable container used to push the enc

5 Putting into operation26 B-390 Operation Manual, Version C5.7.1 Installation of the pressure bottle1. Assemble and – if needed - autoclave the pr

5 Putting into operation27 B-390 Operation Manual, Version C5.8 Option: Concentric nozzle systemThe concentric nozzle system (CN system) is an optio

5 Putting into operation28 B-390 Operation Manual, Version CFigure 5-13: CN bead producing unit with set of 7 shell nozzles. The following nozzle ape

5 Putting into operation29 B-390 Operation Manual, Version C5.8.1 Mounting of CN nozzles12Put the O-ring 12.42×1.78 in the grove of the CN bead prod

Table of contents3 B-390 Operation Manual, Version CRead this manual carefully before installing and running your system and note the safety precautio

5 Putting into operation30 B-390 Operation Manual, Version C Attach the complete CN bead producing unit to the carrier plate with two screws (M3×25).

6 Operation31 B-390 Operation Manual, Version C6 OperationThis chapter gives examples of typical instrument applications and instructions on how to o

6 Operation32 B-390 Operation Manual, Version CThe frequency regulation generates the appropriate elec-tric oscillation in the vibration unit. Pressin

6 Operation33 B-390 Operation Manual, Version CThe light intensity of the stroboscope lamp and amplitude ( = intensity) of the vibration can be set fr

6 Operation34 B-390 Operation Manual, Version C6.4 Manual air pressure controlIn the control unit the pressure is manually controlled by the pressure

6 Operation35 B-390 Operation Manual, Version C6.5 Practicing with the Encapsulator, using waterBefore working with encapsulation polymers, use water

6 Operation36 B-390 Operation Manual, Version CTable 6-1: Determination of the working field (with pressure bottle)Nozzle size:Air pressure Clear bead

6 Operation37 B-390 Operation Manual, Version C7. Set the liquid flow rate and the vibration frequency to a value where a clear bead chain is obtained

6 Operation38 B-390 Operation Manual, Version C6.6 Practicing with the Encapsulator, using alginate solutionAfter getting comfortable with the bead f

6 Operation39 B-390 Operation Manual, Version C6.6.2 Working with alginate solution1. Attach a 200 µm or 300 µm nozzle to the assembled bead produci

Table of contents4 B-390 Operation Manual, Version C6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

6 Operation40 B-390 Operation Manual, Version CNOTEThe stronger the circular dispersal of the bead stream the better is the bead homogeneity. This doe

6 Operation41 B-390 Operation Manual, Version C6.7 Theoretical backgroundEqu. 1: When a laminar jet is mechanically disturbed at the frequency ƒ, bea

6 Operation42 B-390 Operation Manual, Version CFigure 6-4: Influence of the liquid jet velocity and the nozzle diameter on flow rate, as calculated by E

6 Operation43 B-390 Operation Manual, Version CTable 6-4: Optimal working conditions for the Encapsulator determined with alginate solutionNozzle diam

6 Operation44 B-390 Operation Manual, Version CFigure 6-6: Amount of beads with a diameter of 0.3 to 0.6 mm formed from 1 mL of immobilization mixture

6 Operation45 B-390 Operation Manual, Version CFigure 6-8: Amount of cells per bead made from different cell concentrations for bead diameters of 0.3

7 Maintenance and repairs46 B-390 Operation Manual, Version C7 Maintenance and repairsThis chapter gives instructions on maintenance work to be perf

7 Maintenance and repairs47 B-390 Operation Manual, Version C7.4 Cleaning! WarningPressure increase in the inlet-system due to clogged nozzles.Burst

7 Maintenance and repairs48 B-390 Operation Manual, Version CFigure 7-1: Cleaning procedure of the nozzle – Take a syringe containing air on top and

8 Troubleshooting49 B-390 Operation Manual, Version C8 Troubleshooting8.1 Malfunctions and their remedyThe table below lists possible operating erro

1 About this manual5 B-390 Operation Manual, Version C1 About this manualThis manual describes the Encapsulator B-390. It provides all information r

9 Shutdown, storage, transport and disposal50 B-390 Operation Manual, Version C9 Shutdown, storage, transport and disposalThis chapter instructs how

9 Shutdown, storage, transport and disposal51 B-390 Operation Manual, Version C9.2 DisposalFor instrument disposal in an environmentally friendly ma

10 Declarations and requirements52 B-390 Operation Manual, Version C10 Declarations and requirements10.1 FCC requirements (for USA and Canada)Engli

10 Declarations and requirements53 B-390 Operation Manual, Version C10.2 Health and Safety Clearance form Health and Safety Clearance Health and Sa

10 Declarations and requirements54 B-390 Operation Manual, Version C10.3 Declaration of conformity

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2 Safety6 B-390 Operation Manual, Version C2 SafetyThis chapter points out the safety concept of the instrument and contains general rules of behavio

2 Safety7 B-390 Operation Manual, Version C2.4 Safety warnings and safety signals used in this manualDANGER, WARNING, CAUTION and NOTICE are standard

2 Safety8 B-390 Operation Manual, Version CExplosive gases, explosive environmentHarmful to live-formsDevice damagePressurized gas/airHot SurfaceWear

2 Safety9 B-390 Operation Manual, Version C2.5 Product safetySafety warnings in this manual (as described in section 2.4) serve to make the user aler